How far is Key Largo, FL from Gainesville, FL?
The driving distance from Key Largo, FL to Gainesville, FL is about 397.1 miles (639.1 km), with a travel time of about 06h 12m by car.
- The straight-line flight distance is about 335.8 miles (540.4 km).
- For a round trip, plan for roughly 12h 24m of driving time before adding stops, traffic, or weather delays.
- A comfortable departure window is usually 6:00 AM to 7:30 AM EDT.
Reverse, save, or send this route
This drive is better planned with a longer stop or an overnight stay. Copy the route link, send it to another device, or print a clean version before you head out.
This drive is better planned with a longer stop or an overnight stay.
Flight Distance
Driving Distance
Gas Cost Calculator
Leave and Arrive Planner
Pick a departure time to see your estimated arrival and same-day return window.
Drive time stays fixed at 06h 12m.
Starting early still helps, but most travelers will want a longer stop or an overnight stay on a route this long.
You are leaving earlier than the recommended window, which gives you extra margin for breaks and traffic.
Halfway Point
A useful midpoint for breaks, coffee, or meeting in the middle.
Census-designated place in Indian River County, Florida
Route Weather Snapshot
Check the forecast at both ends before you head out.
Mostly Sunny
Partly Sunny
Driving Route Map
The map below follows the saved road route between Key Largo and Gainesville.
Turn-by-Turn Driving Directions
Step-by-step road directions between Key Largo and Gainesville.
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1
Start on Harbor Drive
94 m · 16 sec · Harbor Drive
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2
Turn right onto Overseas Highway
9.3 mi · 16 min · Overseas Highway
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3
Continue on Dixie Highway
19.4 mi · 27 min · Dixie Highway
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4
Take the exit slight right toward Miami, Orlando, Florida's Turnpike North
1785 ft · 38 sec
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5
Continue on Homestead Extension of Florida's Turnpike
13.2 mi · 16 min · Homestead Extension of Florida's Turnpike
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6
Keep slight right to continue on Homestead Extension of Florida's Turnpike
3.6 mi · 5 min · Homestead Extension of Florida's Turnpike
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7
Keep slight left to continue on Homestead Extension of Florida's Turnpike
2.5 mi · 3 min · Homestead Extension of Florida's Turnpike
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8
Keep slight right to continue on Homestead Extension of Florida's Turnpike
27.1 mi · 30 min · Homestead Extension of Florida's Turnpike
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9
Keep slight right to continue on Homestead Extension of Florida's Turnpike
1 mi · 1 min · Homestead Extension of Florida's Turnpike
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10
Merge slight left onto Florida's Turnpike
260.5 mi · 4 hr 43 min · Florida's Turnpike
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11
Keep slight left to continue on Florida's Turnpike
1281 ft · 16 sec · Florida's Turnpike
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12
Merge slight left
46.1 mi · 49 min
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13
Take the exit slight right toward CR 234: Micanopy
1213 ft · 32 sec
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14
Turn right onto Southeast County Road 234
1.3 mi · 2 min · Southeast County Road 234
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15
Turn left
128 m · 15 sec
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16
Turn straight
8 mi · 10 min
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17
Turn right onto Southwest Williston Road
2889 ft · 1 min · Southwest Williston Road
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18
Turn left onto South Main Street
1.6 mi · 3 min · South Main Street
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19
Enter the roundabout and take exit 2 toward South Main Street
28 m · 2 sec · South Main Street
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20
Exit the roundabout onto South Main Street
1317 ft · 32 sec · South Main Street
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21
Enter the roundabout and take exit 2 toward South Main Street
54 m · 4 sec · South Main Street
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22
Exit the roundabout onto South Main Street
2512 ft · 1 min · South Main Street
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23
Arrive at your destination
0 m · 1 sec · South Main Street
Car Route Details
A richer read on the saved driving route between Key Largo and Gainesville.
Long-distance drive
This route is long enough that fuel, rest stops, weather, and timing matter more than any single maneuver.
Florida's Turnpike
Merge slight left onto Florida's Turnpike
261 mi · 04h 43m
Route Summary
A quick read on whether this route feels easy, ambitious, or worth planning around.
Better with a longer stop
This route is long enough that most travelers will be better off planning an overnight stay or a more deliberate stop window.
Mostly Sunny leaving Key Largo and Partly Sunny near Gainesville.
Trip Questions
Direct answers travelers usually want before committing to the drive.
How long does it take to drive from Key Largo to Gainesville?
The road trip is about 397.1 miles (639.1 km) and usually takes 06h 12m in normal driving conditions.
Is this a same-day trip or an overnight route?
This route is long enough that most travelers will be better off planning an overnight stay or a more deliberate stop window.
What is the halfway point on this route?
West Vero Corridor is a useful midpoint, sitting about 198.3 miles from Key Largo and 198.8 miles from Gainesville.
How much longer is the road route than the straight-line distance?
The direct path is about 335.8 miles (540.4 km), while the road route adds roughly 61.3 extra miles because roads do not follow the straight air line.
When is the best time to leave for this route?
A good departure window is usually 6:00 AM to 7:30 AM EDT. Starting early still helps, but most travelers will want a longer stop or an overnight stay on a route this long.
How much will gas cost for this route?
Using about 28.3 MPG, regular gas is estimated at $58.11 one way or $116.23 round trip. Estimated CO2 is about 124.5 kg one way.
What should you check before leaving?
Before you leave, expect Mostly Sunny around Key Largo and Partly Sunny near Gainesville.
Stop Ideas Near West Vero Corridor
Useful break, food, and waypoint ideas within about 15 miles of the route midpoint.
Transit and Meeting Points
Useful pickup, handoff, or rendezvous points close to West Vero Corridor.
Okeechobee
Sebring
West Palm Beach
Kissimmee
Flight Path Map
This map shows the direct air route between Key Largo and Gainesville. Straight-line distance: 335.78 mi (540.39 km).
#1 Key Largo
Census designated place in Florida, United States
| Population | 10,433 |
| Coordinates | 25.0865200, -80.4472800 |
| Altitude | 2 |
| Country | United States |
#2 Gainesville
The third most populous city in Florida.
| Population | 135,004 |
| Coordinates | 29.6516300, -82.3248300 |
| Altitude | 54 |
| Country | United States |
End: South Main Street (-82.32501, 29.65163)
Estimated Travel Time
Distance: 639 km by road
| Speed | Time |
|---|---|
| 30 mph (48.3 km/h) | 13h 13m |
| 40 mph (64.37 km/h) | 09h 55m |
| 50 mph (80.47 km/h) | 07h 56m |
| 60 mph (96.56 km/h) | 06h 37m |
| 70 mph (112.65 km/h) | 05h 40m |
| 80 mph (129 km/h) | 04h 57m |
Fuel, Round Trip and CO2
Estimated fuel cost and emissions for this route.
| Fuel | $/gal | One way | Round trip |
|---|---|---|---|
| Regular gas | $4.15 | $58.11 | $116.23 |
| Mid-grade | $4.60 | $64.39 | $128.78 |
| Premium | $4.90 | $68.59 | $137.19 |
| Diesel | $4.35 | $60.94 | $121.89 |
Assumes 8.3 L/100 km (about 28.3 MPG). CO2 is an estimate and varies by vehicle, fuel blend, traffic, and terrain.
Prices in Florida · Apr 23, 2026
Distance to Other Cities
Planning a road trip? See the full trip guide on Trip.ovh — stops, fuel costs, weather, and departure timing.