| # | Name | Population |
| 501 | Ponta Delgada, municipality and city in Portugal | 68,748 |
| 502 | L Aquila, Italian city, capital of the Abruzzo region and of the Province of L'Aquila | 69,439 |
| 503 | Calarasi, city in Călărași County, Romania | 70,039 |
| 504 | Vaslui, city in Vaslui County, Romania | 70,267 |
| 505 | Karakol, city in Kyrgyzstan | 70,400 |
| 506 | Halmstad, urban area in Halmstad Municipality, Sweden | 70,480 |
| 507 | Vaxjo, Urban area in Växjö Municipality, Sweden | 70,489 |
| 508 | Ajaccio, commune in Corse-du-Sud department, Corsica, France | 70,659 |
| 509 | Kumanovo, city in North Macedonia | 70,842 |
| 510 | Mamoudzou, commune in Mayotte, France | 71,437 |
| 511 | Santa Fe, county seat city in Santa Fe County, New Mexico, USA and New Mexico federated state capital city | 71,831 |
| 512 | Abancay, capital city of Apurímac, Peru | 72,277 |
| 513 | Zadar, county seat city in Zadar County, Croatia | 72,718 |
| 514 | Szolnok, county seat of Jász-Nagykun-Szolnok county in central Hungary | 73,193 |
| 515 | Resita, city in Caraș-Severin County, Romania | 73,282 |
| 516 | Bismarck, capital of North Dakota, United States and seat of Burleigh County | 73,529 |
| 517 | Giurgiu, city in Giurgiu County, Romania | 73,586 |
| 518 | Birobidzhan, town and administrative center of the Jewish Autonomous Oblast in the far east of Russia | 73,623 |
| 519 | Kasama, city in Ibaraki Prefecture, Japan | 74,334 |
| 520 | Bitola, city in North Macedonia | 74,550 |
| 521 | Nitra, city in Slovakia | 77,374 |
| 522 | Szombathely, city in Hungary | 77,566 |
| 523 | Gavle, urban area in Gävle Municipality, Sweden | 77,699 |
| 524 | Lelystad, municipality and capital city of Flevoland, Netherlands | 77,893 |
| 525 | Banska Bystrica, city in central Slovakia | 78,327 |
| 526 | Aveiro, municipality and city in Portugal | 78,450 |
| 527 | Slatina, city in Olt County, Romania | 78,815 |
| 528 | Fort de France, Commune in Martinique, France | 80,041 |
| 529 | Luzern, city on Lake Lucerne, in central Switzerland | 81,691 |
| 530 | Trenton, capital of New Jersey, United States and seat of Mercer County, New Jersey, United States | 83,203 |
| 531 | Yambol, city in Bulgaria | 84,232 |
| 532 | Honiara, capital of Solomon Islands, on the tropical island Guadalcanal | 84,520 |
| 533 | Santa Fe, county seat city in Santa Fe County, New Mexico, USA and New Mexico federated state capital city | 84,683 |
| 534 | Ceuta, autonomous city of Spain in North Africa | 84,777 |
| 535 | Puerto Maldonado, capital city of Madre de Dios, Peru | 85,024 |
| 536 | Piatra Neamt, city in Neamț County, Romania | 85,055 |
| 537 | Melilla, Autonomous city of Spain in North Africa | 86,487 |
| 538 | Saki, city in Gifu Prefecture, Japan | 86,698 |
| 539 | Poitiers, commune in Vienne, France | 88,291 |
| 540 | Bertoua, commune of Cameroon | 88,462 |
| 541 | Panevezys, city in Lithuania | 88,678 |
| 542 | Presov, city in Slovakia | 88,680 |
| 543 | Viana do Castelo, municipality and city in Portugal | 88,725 |
| 544 | Umea, urban area in Umeå Municipality, Sweden | 89,607 |
| 545 | Catanzaro, Italian city and capital of the Calabria province | 89,718 |
| 546 | Targoviste, city in Dâmbovița County, Romania | 89,773 |
| 547 | Balkanabat, human settlement | 89,785 |
| 548 | Pardubice, city in the Czech Republic | 90,335 |
| 549 | Tulcea, city in Tulcea County, Romania | 91,875 |
| 550 | Drobeta Turnu Severin, city in Mehedinți County, Romania | 92,617 |
| 551 | Tartu, second largest city of Estonia | 92,974 |
| 552 | Schwerin, capital city of Mecklenburg-Vorpommern, Germany | 95,818 |
| 553 | Noumea, capital city of the French special collectivity of New Caledonia | 96,082 |
| 554 | Hradec Kralove, city in the Czech Republic | 96,145 |
| 555 | Albany, capital of New York, United States and seat of Albany County | 96,460 |
| 556 | Targu Jiu, city in Gorj County, Romania | 96,641 |
| 557 | Szekesfehervar, city in Hungary | 96,940 |
| 558 | Ceske Budejovice, city in the Czech Republic | 97,243 |
| 559 | Santiago de Compostela, city in north-west Spain in the La Coruña province | 97,260 |
| 560 | Jonkoping, urban area in Jönköping Municipality, Sweden | 99,373 |
| 561 | Khanty Mansiysk, city in eastern Russia | 99,385 |
| 562 | Usti nad Labem, 9th-most populous city of the Czech Republic, capital of its eponymous region and district | 100,002 |
| 563 | Olomouc, city in the Czech Republic | 100,154 |
| 564 | Siauliai, city in Lithuania | 100,575 |
| 565 | Ancona, Italian city in the Marches region. | 100,924 |
| 566 | Drammen, municipality in Viken, Norway | 101,386 |
| 567 | Klagenfurt, capital city of Carinthia, Austria | 101,403 |
| 568 | Focsani, city in Vrancea County, Romania | 101,854 |
| 569 | Satu Mare, city in Satu Mare County, Romania | 102,411 |
| 570 | Elista, city in the south of Russia | 103,132 |
| 571 | Liberec, Czech city | 103,288 |
| 572 | Caen, commune in Calvados, France | 105,354 |
| 573 | Suceava, city in Suceava County, Romania | 105,865 |
| 574 | Ramnicu Valcea, city in Vâlcea County, Romania | 107,726 |
| 575 | Tabuk, region of Saudi Arabia | 109,940 |
| 576 | Bridgetown, capital of Barbados | 110,000 |
| 577 | Rouen, commune in Seine-Maritime, France | 110,145 |
| 578 | Saint Denis, commune in Seine-Saint-Denis, Paris agglomeration, France | 111,135 |
| 579 | Kecskemet, city in the central part of Hungary | 112,071 |
| 580 | Lansing, city in Ingham County, Eaton County and Clinton County, Michigan, USA and Michigan federated state capital city | 113,328 |
| 581 | Alborg, city in Denmark | 113,417 |
| 582 | Linkoping, urban area in Linköping Municipality, Sweden | 114,582 |
| 583 | Uzhhorod, city and administrative center of Zarkapattia Oblast and Uzhorod District in western Ukraine | 114,897 |
| 584 | Botosani, city in Botoșani County, Romania | 115,070 |
| 585 | Besancon, commune in Doubs, France | 115,934 |
| 586 | Metz, commune in Moselle, France | 116,429 |
| 587 | Orleans, city in Centre-Val de Loire, France | 116,685 |
| 588 | Kyzyl, city in eastern Russia | 116,983 |
| 589 | Trento, Italian city in the North-East of the country | 117,997 |
| 590 | Nyiregyhaza, city in Hungary | 118,164 |
| 591 | Huaraz, city of Peru, capital of Ancash region | 118,836 |
| 592 | Maastricht, municipality in the Netherlands | 121,565 |
| 593 | Gyumri, second-largest city in Armenia and capital of Shirak Province in the northwestern part of the country | 121,976 |
| 594 | Hartford, city in and capital of Connecticut, United States | 122,105 |
| 595 | Cherkessk, city in the south of Russia | 122,395 |
| 596 | Punta Arenas, Chilean city beside the Strait of Magellan | 123,403 |
| 597 | Orebro, urban area in Örebro Municipality, Sweden | 125,817 |
| 598 | Koforidua, capital city of eastern region | 127,334 |
| 599 | Opole, city with powiat rights of Poland | 127,792 |
| 600 | Vasteras, urban area in Västerås Municipality, Sweden | 127,799 |